ASEAN Journal on Science and Technology for Development
Abstract
A strain of Bacillus subtilis FS2 isolated from a traditional fish source in Vietnam has been shown to produce an enzyme possessing both a gelatinase and a lipase A (LipA) activity when tested on agar plates containing 0.1% tributyrin and 0.3% gelatine. A gene encoding LipA was isolated and expressed in E. coli BL21 (DE3). The enzyme has got an apparent Mr of 24 kDa, a pI of 9.2 and was purified to electrophoretical homogeneity by affinity chromatography. Both the lipase and the gelatinase showed optimum activity at pH 10 and at 30 0 C, with highest activity in the range 25-37°C. The metal ions Fe++, Co++, and Mn++ at 10 mM had strong inhibitory effect on both activities whereas Ca++ had no effect and Zn++ had a slight enhancing effect. The specific enzymatic activities obtained were 19814 U/mg for the lipase and 1245 U/mg for the gelatinase.
Publication Date
11-22-2017
Recommended Citation
Thu, Nguyet Phung; Hong, Thanh Nguyen; Nam, Hai Truong; and Jan-Christer, Janson
(2017)
"STUDIES ON A NOVEL RECOMBINANT LIPASE A FROM BACILLUS SUBTILIS FS2,"
ASEAN Journal on Science and Technology for Development: Vol. 25:
No.
2, Article 17.
DOI: https://doi.org/10.29037/ajstd.263
Available at:
https://ajstd.ubd.edu.bn/journal/vol25/iss2/17