R unning Head: Novel Recombinant Phytate - d egrading Enzyme Production Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology

Authors

Farouk Abd #NAME?
Meor Hussin Anis Shobirin
Greiner Ralf
Ismail Shareef Mohideen
Batcha Mohamed Faizal
Nur Lubis Hamadah Mohd

Abstract

The production of phytase in a recombinant E.coliusing thepBAD expression system wasoptimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentrationin the cultivation media and the incubation temperature were optimized in order to maximize phytase productionusing 23 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among thesefactors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. OPtim al conditionsfor phytase production were determinedto be100 μg/ml ampicilin, 0.2 % arabinose and an incubation temperature of37ºC.The production of phytase in the recombinant E. coliwas scaledup to 100ml and 1000ml.

Publication Date

11-12-2017

Recommended Citation

#NAME?, Farouk Abd; Shobirin, Meor Hussin Anis; Ralf, Greiner; Mohideen, Ismail Shareef; Faizal, Batcha Mohamed; and Mohd, Nur Lubis Hamadah (2017) "R unning Head: Novel Recombinant Phytate - d egrading Enzyme Production Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology," ASEAN Journal on Science and Technology for Development: Vol. 26: No. 1, Article 8.
DOI: https://doi.org/10.29037/ajstd.179
Available at: https://ajstd.ubd.edu.bn/journal/vol26/iss1/8