ASEAN Journal on Science and Technology for Development


Medicinal plants are generally known for its health benefits and there has been ongoing research to gather evidence on potential plants that could exhibit antioxidant activities. In this study, we explored underutilised local medicinal plants which are Stenochlaena palustris and Diplazium esculentum, locally known as ‘Lemiding’ and ‘Pakis’, respectively. The aim was to determine the effect of extraction time on phytochemical contents, antioxidant activities and total phenolic content (TPC) of the two plant species. Maceration method was used to extract the compounds from leaves of the plant in distilled water at different durations of extraction i.e. 2 hours, 4 hours, 6 hours, 8 hours and 24 hours. Phytochemical screening of the two plant species as aqueous extracts detected the presence of reducing sugar, phenol and saponins. DPPH radical scavenging antioxidant assay revealed that 8-hour S. palustris aqueous extracts (77.6 ± 0.04%) and 2-hour D. esculentum aqueous extracts (48.8 ± 0.05%) exhibited the highest radical scavenging activities in each plant species. Additionally, quantitative results of phenolic compounds were found to be twice as high in S. palustris (11.27± 2.44) than in D. esculentum (4.34 ± 0.95) aqueous extracts. Based on these findings, S. palustris emerges as a more promising antioxidant, highlighting its role for potential applications.

Publication Date


Received Date

14-September- 2023

Revised Date


Accepted Date